| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peters, Mallory (13)
|
ADWAT |
59.17 |
58.99 |
-0.18 |
| 2 |
LaDue, Rose (14)
|
FUL |
1:00.14 |
59.96 |
-0.18 |
| 3 |
Burroughs, Ellen A (14)
|
NIAYST |
59.94 |
1:00.25 |
0.31 |
| 4 |
Comer, Cate E (14)
|
NIAYST |
57.53 |
1:00.53 |
3.00 |
| 5 |
Springer, Elizabeth R (14)
|
NIAYST |
56.95 |
1:01.93 |
4.98 |
| 6 |
Soprano, Sophia (13)
|
NISKAN |
1:03.25 |
1:02.26 |
-0.99 |
| 7 |
Jarvie, Aurora (14)
|
ADWAT |
1:03.64 |
1:02.77 |
-0.87 |
| 8 |
Brown, Chloe (14)
|
NIAYST |
1:01.91 |
1:03.90 |
1.99 |
| 9 |
Dempsey, Gracie (14)
|
FUL |
1:08.82 |
1:04.91 |
-3.91 |
| 10 |
Buchholz, Marcella C (14)
|
NIAYST |
1:03.86 |
1:05.58 |
1.72 |
| 11 |
Lattimore-McDermott, Kara (13)
|
NIAYST |
1:03.53 |
1:05.65 |
2.12 |
| 12 |
Truesdell, Briegha (14)
|
ADONFY |
1:05.79 |
1:06.38 |
0.59 |
| 13 |
DeProspero, Stephanie E (14)
|
NIAYST |
1:07.08 |
1:08.47 |
1.39 |
| 14 |
Smith, Ava B (14)
|
NIAYST |
1:07.92 |
1:08.64 |
0.72 |
| 15 |
Funk, Meghan (13)
|
ADNDYS |
1:14.24 |
1:11.56 |
-2.68 |
| 16 |
Kelsey, Madison E (13)
|
NICORY |
1:11.22 |
1:12.87 |
1.65 |
| 17 |
Bott, Madison G (13)
|
NICORY |
1:10.18 |
1:12.89 |
2.71 |
| 18 |
Omans, Bethany M (13)
|
NISKAN |
1:09.73 |
1:13.80 |
4.07 |
| 19 |
Barbeau, Hailey (13)
|
FUL |
1:15.32 |
1:14.39 |
-0.93 |
| 20 |
Scott, Kaylyn (14)
|
ADWAT |
1:14.92 |
1:14.45 |
-0.47 |
| 21 |
Eastman, Beth (14)
|
ADWAT |
1:18.83 |
1:16.00 |
-2.83 |
| 22 |
Sanchez, Melaina (14)
|
ADWAT |
1:16.19 |
1:16.82 |
0.63 |
| 23 |
Delano, Madelyn (14)
|
FUL |
1:20.15 |
1:19.36 |
-0.79 |
| 24 |
Rodriguez, Mia (13)
|
FUL |
1:20.57 |
1:22.42 |
1.85 |
| 25 |
Baxter, Annabeth (14)
|
ADWAT |
1:23.38 |
1:25.54 |
2.16 |
| 26 |
Fraleigh-Barrows, Lillian P (13)
|
NICORY |
1:34.30 |
1:30.46 |
-3.84 |
| 27 |
Evans, Molly (13)
|
FUL |
1:37.50 |
1:41.14 |
3.64 |
|
Kelly, Kennedy M (13)
|
ADONEI |
1:33.48 |
NS |
- |