| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Oushalkas, Anna K (10)
|
NTTFA |
31.78 |
31.70 |
-0.08 |
| 2 |
Southern, Kennedi H (10)
|
NTLAC |
34.28 |
33.25 |
-1.03 |
| 3 |
Rogers, Drue E (9)
|
NTLAC |
34.52 |
33.90 |
-0.62 |
| 4 |
Ducayet, Marie Therese C (10)
|
NTCOR |
36.22 |
34.24 |
-1.98 |
| 5 |
Wang, Yeyi (9)
|
NTLAC |
34.92 |
34.61 |
-0.31 |
| 6 |
Dowell, Lila G (10)
|
NTLAC |
35.37 |
34.66 |
-0.71 |
| 7 |
Ware, Haley V (10)
|
NTTFA |
34.53 |
34.75 |
0.22 |
| 8 |
Valko, Karolina A (10)
|
NTDM |
34.31 |
35.29 |
0.98 |
| 9 |
Salmassian, Christine K (9)
|
NTDM |
36.37 |
35.57 |
-0.80 |
| 10 |
Potter, Tinsley G (10)
|
NTLAC |
36.39 |
36.73 |
0.34 |
| 11 |
Pan-Wang, Alina (9)
|
NTLAC |
35.96 |
37.19 |
1.23 |
| 12 |
Schnapp, Lainey M (9)
|
NTLAC |
36.38 |
37.72 |
1.34 |