| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chaney, Lauren E (17)
|
STNTRO |
5:01.12 |
5:00.51 |
-0.61 |
| 2 |
Thome, Allie F (16)
|
GUKATY |
4:59.51 |
5:05.84 |
6.33 |
| 3 |
Krantzcke, Isabella G (15)
|
GUTWST |
4:55.57 |
5:09.60 |
14.03 |
| 4 |
Hardy, Abigail (15)
|
STNTRO |
5:25.21 |
5:14.99 |
-10.22 |
| 5 |
Lancaster, Thea A (17)
|
STATAC |
5:06.83 |
5:15.30 |
8.47 |
| 6 |
Filimoehala, Trisha T (16)
|
STNTRO |
5:11.37 |
5:23.06 |
11.69 |
| 7 |
Barley, Ariel B (16)
|
STATAC |
5:24.64 |
5:26.08 |
1.44 |
| 8 |
Weiss, Madison R (15)
|
STASC |
5:16.67 |
5:28.50 |
11.83 |
| 9 |
Bose, Ariana M (18)
|
STATAC |
5:38.60 |
5:31.70 |
-6.90 |
| 9 |
Monroe, Libby (15)
|
GUMAC |
5:49.82 |
5:31.70 |
-18.12 |
| 11 |
Norris, Grace L (15)
|
GUBATS |
5:17.90 |
5:33.37 |
15.47 |
| 12 |
Wilkins, Chloe E (15)
|
STNTRO |
6:01.66 |
5:36.44 |
-25.22 |
| 13 |
Weeks, Sonya C (15)
|
STATAC |
5:48.92 |
5:46.90 |
-2.02 |
| 14 |
Chow, Priscilla C (15)
|
STASC |
|
7:12.54 |
- |