| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dixon, Madelyn M (8)
|
MDHOCO |
40.76 |
38.16 |
-2.60 |
| 2 |
Weber, Ella L (8)
|
MDSPRC |
38.84 |
38.97 |
0.13 |
| 3 |
Tomlinson, Sophia G (8)
|
MDFSC |
42.88 |
42.14 |
-0.74 |
| 4 |
Stutz, Caroline G (8)
|
MDSPRC |
39.25 |
42.36 |
3.11 |
| 5 |
Ohlinger, Danielle S (8)
|
MDFSC |
43.43 |
43.87 |
0.44 |
| 6 |
Holder, LeeAnn M (8)
|
MDHOCO |
|
45.13 |
- |
| 7 |
Herbein, Hanna E (8)
|
MDACA |
45.77 |
45.49 |
-0.28 |
| 8 |
Mueller, Sophia E (8)
|
MDFAST |
|
47.55 |
- |
| 9 |
Stafford, Harper S (8)
|
MDFSC |
1:00.23 |
48.22 |
-12.01 |
| 10 |
Fondale, Elaine A (8)
|
MDASC |
|
50.60 |
- |
| 11 |
Mets, Anna (7)
|
MDMAS |
50.60 |
51.10 |
0.50 |
| 12 |
Devine, Madeline G (7)
|
MDFSC |
49.47 |
51.68 |
2.21 |
| 13 |
Mahl, Siena M (7)
|
MDSPRC |
|
58.54 |
- |
| 14 |
Tate, Emerson G (8)
|
MDFSC |
|
1:05.45 |
- |
| 15 |
Estes, Isabella L (8)
|
MDSPRC |
|
1:11.22 |
- |
| 16 |
King, Josephine J (8)
|
MDASC |
|
1:11.45 |
- |
|
Zhu, Emily Y (6)
|
MDHOCO |
|
DQ |
- |
|
Francis, Ava G (8)
|
MDROCK |
|
DQ |
- |
|
FitzGerald, Elizabeth J (8)
|
MDFSC |
|
DQ |
- |
|
Agusto-Cox, Katerina A (8)
|
MDFSC |
|
DQ |
- |
|
Kohl, Genevieve W (8)
|
MDCAC |
|
DQ |
- |
|
Haugh, Michelle E (8)
|
MDCAC |
|
DQ |
- |