| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yang, Leo (17)
|
VAHOKI |
47.27 |
47.28 |
0.01 |
| 2 |
Nester, Jackson C (17)
|
VAHOKI |
50.40 |
50.08 |
-0.32 |
| 3 |
Zolovick, Nicholas A (16)
|
VAHOKI |
52.05 |
53.98 |
1.93 |
| 4 |
Rezac, Tyler E (14)
|
VAHOKI |
56.45 |
56.22 |
-0.23 |
| 5 |
Nussbaum, Jack I (14)
|
VAHOKI |
55.49 |
56.28 |
0.79 |
| 6 |
Reilly, Samuel J (14)
|
VAHOKI |
54.51 |
56.32 |
1.81 |
| 7 |
Hollandsworth, Trent J (13)
|
VAHOKI |
56.43 |
56.36 |
-0.07 |
| 8 |
Goldstein, Nicholas D (16)
|
VAHOKI |
58.22 |
57.83 |
-0.39 |
| 9 |
King, Kolby G (16)
|
VAHOKI |
57.20 |
58.32 |
1.12 |
| 10 |
Zhao, Ryan (14)
|
VAHOKI |
1:06.19 |
1:05.18 |
-1.01 |
| 11 |
Stark, Jacob R (14)
|
VASCAT |
1:11.88 |
1:09.93 |
-1.95 |