| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DaCosta, Rafael L (18)
|
WIECY |
47.41 |
49.76 |
2.35 |
| 2 |
Dickman, Wyatt L (16)
|
WIWYNS |
50.08 |
50.97 |
0.89 |
| 3 |
Sande, Austin M (18)
|
WIECY |
49.97 |
51.51 |
1.54 |
| 4 |
Sperl, Luke A (14)
|
WISPDY |
51.66 |
52.09 |
0.43 |
| 5 |
Lanaville, Nathaniel (13)
|
DCNLY |
54.51 |
52.56 |
-1.95 |
| 6 |
Merkatoris, Thomas J (15)
|
WIECY |
51.54 |
52.62 |
1.08 |
| 7 |
Frazier, Jaeger R (16)
|
WIWYNS |
51.52 |
52.69 |
1.17 |
| 8 |
Bowman, Simon S (14)
|
WILAXY |
54.48 |
54.14 |
-0.34 |
| 9 |
Toufar, Alexander J (15)
|
WIECY |
56.70 |
54.41 |
-2.29 |
| 10 |
Harrison, Evan (13)
|
WIMTRY |
55.37 |
54.45 |
-0.92 |
| 11 |
Rineck, Rowan (15)
|
WICVY |
1:04.38 |
55.47 |
-8.91 |
| 12 |
Sande, Mason F (14)
|
WIECY |
57.61 |
56.54 |
-1.07 |
| 13 |
Krause, Joshua D (14)
|
WIECY |
1:08.87 |
58.09 |
-10.78 |
| 14 |
Beatty, Blake C (12)
|
WIWYNS |
57.72 |
58.53 |
0.81 |
| 15 |
McKinley, Keegan (15)
|
WISPDY |
1:00.84 |
58.77 |
-2.07 |
| 16 |
Rossi, Jacob N (13)
|
WIECY |
59.22 |
59.15 |
-0.07 |
| 17 |
Van Ermen, William J (11)
|
WIWYNS |
1:00.21 |
1:00.85 |
0.64 |
| 18 |
Tichy, Cole (14)
|
WIMYST |
1:00.79 |
1:02.32 |
1.53 |
| 19 |
Regan, Harrison O (12)
|
WILAXY |
1:04.58 |
1:03.23 |
-1.35 |
| 20 |
Soik, Mason (12)
|
WISPDY |
1:04.50 |
1:03.27 |
-1.23 |
| 21 |
Getzloff, Colten (13)
|
DCNLY |
1:04.16 |
1:05.56 |
1.40 |
| 22 |
Fuse, Weston A (13)
|
WIECY |
1:04.26 |
1:07.08 |
2.82 |
| 23 |
Stargardt, Dakota (13)
|
WIMTRY |
1:07.25 |
1:07.65 |
0.40 |
| 24 |
O'Donnell, Sean P (13)
|
WIWYNS |
1:11.44 |
1:08.33 |
-3.11 |
| 25 |
Soehl, Benjamin N (12)
|
WIWYNS |
1:09.30 |
1:09.71 |
0.41 |
| 26 |
Polley, Wes J (11)
|
WIWYNS |
1:13.13 |
1:12.64 |
-0.49 |
| 27 |
Hoffman, Conner (12)
|
WISPDY |
1:31.34 |
1:21.59 |
-9.75 |
| 28 |
Jelen, Alexander P (11)
|
WIWYNS |
2:07.46 |
1:52.38 |
-15.08 |
|
Milne, Max W (14)
|
WIECY |
1:05.87 |
NS |
- |