| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rommel, Julia E (10)
|
MDNBAC |
32.87 |
31.53 |
-1.34 |
| 2 |
Jiang, Lori (10)
|
MRWEST |
32.76 |
32.52 |
-0.24 |
| 3 |
Hoover, Annabelle M (10)
|
NEWW |
34.95 |
34.13 |
-0.82 |
| 4 |
van Lierop, Catherine (10)
|
MDNBAC |
36.15 |
34.46 |
-1.69 |
| 5 |
Siegel, Alexandra R (9)
|
MRLIAC |
35.98 |
34.75 |
-1.23 |
| 6 |
Geary, Samantha S (9)
|
NJBAC |
35.60 |
34.88 |
-0.72 |
| 7 |
Kim, Haley M (10)
|
MDNBAC |
36.15 |
35.08 |
-1.07 |
| 8 |
Dagorova, Mina N (10)
|
MESMAC |
36.20 |
35.13 |
-1.07 |
| 9 |
Strabuk, Summer R (10)
|
MRLIAC |
36.18 |
35.55 |
-0.63 |
| 10 |
Mesceda, Magdalena M (10)
|
MDNBAC |
36.42 |
36.07 |
-0.35 |
| 11 |
Matteini, Hannah J (10)
|
MDNBAC |
36.49 |
36.29 |
-0.20 |
| 12 |
Kurzatkowski, Victoria (10)
|
MDNBAC |
36.41 |
36.64 |
0.23 |
| 13 |
Krawiec, Chloe V (10)
|
NJBAC |
36.40 |
36.98 |
0.58 |
| 14 |
Katt, Emily (9)
|
NJBAC |
36.43 |
37.92 |
1.49 |