| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tenhundfeld, Emma L (13)
|
OHKEY |
2:42.45 |
2:34.07 |
-8.38 |
| 2 |
Tenhundfeld, Ava J (15)
|
OHKEY |
2:36.16 |
2:35.20 |
-0.96 |
| 3 |
Derringer, Alana N (17)
|
OHSPY |
2:31.20 |
2:38.52 |
7.32 |
| 4 |
Turner, Reese L (17)
|
OHSPY |
2:39.88 |
2:42.93 |
3.05 |
| 5 |
Derr, Penelope F (13)
|
OHSPY |
2:46.60 |
2:46.41 |
-0.19 |
| 6 |
Woolf-Isbel, MacKenzie K (15)
|
OHKEY |
2:42.84 |
2:47.41 |
4.57 |
| 7 |
Wentzel, Hannah R (14)
|
OHKEY |
2:43.63 |
2:48.11 |
4.48 |
| 8 |
Haas, Abigail E (15)
|
OHKEY |
3:04.93 |
2:48.20 |
-16.73 |
| 9 |
Spetz, Corinne E (13)
|
OHLAKY |
3:07.77 |
2:50.41 |
-17.36 |
| 10 |
Smith, Madison E (12)
|
OHKEY |
2:53.12 |
2:52.89 |
-0.23 |
| 11 |
Mitter, Rachel L (14)
|
OHKEY |
|
2:53.38 |
- |
| 12 |
Schneider, Victoria C (13)
|
OHLAKY |
2:46.22 |
2:56.34 |
10.12 |
| 13 |
Crowe, Kayleen A (15)
|
OHLAKY |
2:59.71 |
3:01.02 |
1.31 |
| 14 |
Zeppettella, Carla E (14)
|
OHCFY |
2:54.16 |
3:05.19 |
11.03 |
| 15 |
Crowe, Felicia L (13)
|
OHLAKY |
|
3:15.09 |
- |
| 16 |
Schirg, Aizza M (13)
|
OHUCFY |
3:21.42 |
3:18.85 |
-2.57 |
| 17 |
Bhatti, Angelica (14)
|
OHLAKY |
2:49.07 |
3:27.75 |
38.68 |
| 18 |
Jois, Priyanka R (13)
|
OHLAKY |
3:41.71 |
3:32.31 |
-9.40 |