| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaskill, Lillian J (8)
|
OHCFY |
21.96 |
21.14 |
-0.82 |
| 2 |
Hill, Elizabeth J (8)
|
OHSPY |
20.89 |
21.38 |
0.49 |
| 3 |
Alexander, Emily E (8)
|
OHMCY |
21.45 |
21.58 |
0.13 |
| 4 |
Montgomery, Madeline E (8)
|
OHPCYP |
22.50 |
22.07 |
-0.43 |
| 5 |
Lee, Aubrey B (7)
|
OHSPY |
21.70 |
22.10 |
0.40 |
| 6 |
Stuckey, Allie M (7)
|
OHSPY |
22.36 |
22.99 |
0.63 |
| 7 |
Brown, Brianna E (8)
|
OHPCYP |
23.02 |
23.44 |
0.42 |
| 8 |
Zwick, Lily M (8)
|
OHGMVY |
23.97 |
23.47 |
-0.50 |
| 9 |
Gearhardt, Haley A (8)
|
OHMCY |
22.04 |
23.52 |
1.48 |
| 10 |
Haun, Maci K (8)
|
OHHHY |
22.77 |
23.96 |
1.19 |
| 11 |
Statesman, Addison E (7)
|
OHHHY |
22.22 |
23.97 |
1.75 |
| 12 |
Gatch, Persephone A (8)
|
OHKEY |
23.65 |
24.16 |
0.51 |
| 13 |
Derr, Olympia S (7)
|
OHSPY |
25.58 |
24.32 |
-1.26 |
| 14 |
Marshall, Harper W (7)
|
OHGMVY |
28.78 |
24.49 |
-4.29 |
| 15 |
Saito, Anne M (7)
|
OHKZY |
24.67 |
24.54 |
-0.13 |
| 16 |
Schell, Lilly K (8)
|
OHKZY |
25.52 |
24.62 |
-0.90 |
| 17 |
Elam, Dublin D (7)
|
OHCFY |
25.17 |
24.67 |
-0.50 |
| 18 |
Kirchner, Genevieve A (8)
|
OHCFY |
25.92 |
25.38 |
-0.54 |
| 19 |
Muchow, Kaitlynn M (8)
|
OHKEY |
27.35 |
26.48 |
-0.87 |
| 20 |
Bommer, Harriett C (7)
|
OHCFY |
26.32 |
26.56 |
0.24 |
| 21 |
Niu, Hannah X (7)
|
OHMCY |
29.81 |
27.96 |
-1.85 |
| 22 |
Culver, Isla R (8)
|
OHMCY |
26.98 |
28.28 |
1.30 |
| 23 |
Astor, Hayley G (8)
|
OHMCY |
26.50 |
28.46 |
1.96 |
| 24 |
Haas, Bella E (7)
|
OHKEY |
26.38 |
28.80 |
2.42 |
| 25 |
Mullins, Lillie R (8)
|
OHKZY |
30.51 |
28.95 |
-1.56 |
| 26 |
Couvutsakis, Violet (7)
|
OHPCYP |
30.30 |
29.75 |
-0.55 |
| 27 |
Akers, Chloe M (8)
|
OHKEY |
33.71 |
34.87 |
1.16 |
| 28 |
Moglia, Delaney S (7)
|
OHKEY |
31.36 |
36.48 |
5.12 |
|
Rifki, Ayah (7)
|
OHKZY |
28.37 |
NS |
- |
|
Dillingham, Heather R (8)
|
OHKZY |
43.24 |
NS |
- |
|
Shuping, Virginia R (7)
|
OHSPY |
25.02 |
NS |
- |