| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dearborn, Kali (10)
|
KYNEY |
35.38 |
33.12 |
-2.26 |
| 2 |
Simpson, Kendall A (9)
|
KYAAY |
37.95 |
36.03 |
-1.92 |
| 3 |
Reynolds, Madeline M (10)
|
KYNLY |
36.74 |
36.20 |
-0.54 |
| 4 |
Akridge, Cora (10)
|
KYNEY |
39.49 |
36.55 |
-2.94 |
| 5 |
Geile, Marci G (10)
|
KYTYP |
39.22 |
38.78 |
-0.44 |
| 6 |
Ogata, Ayano (10)
|
KYBYB |
40.77 |
39.07 |
-1.70 |
| 7 |
Kemplin, Meghan (10)
|
KYNEY |
37.75 |
40.42 |
2.67 |
| 8 |
El-Amin-Stewart, Azeezah (10)
|
KYNLY |
41.91 |
41.17 |
-0.74 |
| 9 |
Dever, Lydia (10)
|
KYNLY |
41.30 |
43.14 |
1.84 |
| 10 |
Derbyshire, Gwendolyn W (10)
|
KYNLY |
43.58 |
44.34 |
0.76 |
| 11 |
Ford, Isabelle (10)
|
KYNEY |
46.17 |
45.00 |
-1.17 |
| 12 |
Rafferty, Ella (9)
|
KYNLY |
49.75 |
46.60 |
-3.15 |
| 13 |
Hamilton, Peyton (9)
|
KYNEY |
45.92 |
46.85 |
0.93 |
| 14 |
Knuckles, Katelyn P (9)
|
KYAAY |
51.02 |
47.07 |
-3.95 |
| 15 |
Godthaab, Grace (10)
|
KYNEY |
46.54 |
47.82 |
1.28 |
| 16 |
Martin, Caroline (9)
|
KYNEY |
49.53 |
49.12 |
-0.41 |
| 17 |
Broady, Elisabeth (9)
|
KYNLY |
52.19 |
49.75 |
-2.44 |
| 18 |
Myers, Adelyn (9)
|
KYNLY |
56.08 |
53.41 |
-2.67 |
| 19 |
Weber, Colette R (9)
|
KYBYB |
50.26 |
55.13 |
4.87 |
| 20 |
Kinningham, Lilly C (10)
|
KYBYB |
1:05.97 |
58.24 |
-7.73 |
| 21 |
Harman, Lia A (10)
|
KYBYB |
1:02.51 |
1:00.88 |
-1.63 |
| 22 |
Haines, Sarah (9)
|
KYNEY |
58.51 |
1:01.33 |
2.82 |
| 23 |
Gooch, Stella L (9)
|
KYBYB |
1:11.32 |
1:04.91 |
-6.41 |
| 24 |
Harmon, Savannah (10)
|
KYNLY |
1:06.98 |
1:05.56 |
-1.42 |
| 25 |
Bogale, Lilly I (9)
|
KYBYB |
1:09.92 |
1:08.58 |
-1.34 |
| 26 |
Vaish, Anvi (10)
|
KYBYB |
1:30.70 |
1:41.24 |
10.54 |
|
Hadi, Aya F (9)
|
KYBYB |
1:01.24 |
DQ |
- |