| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holt, Sarah E (18)
|
LAYNWL |
55.21 |
54.34 |
-0.87 |
| 2 |
Gill, Ashley M (17)
|
LACOLA |
56.39 |
56.66 |
0.27 |
| 3 |
Janszen, Amanda M (33)
|
LAYNWL |
58.36 |
58.19 |
-0.17 |
| 4 |
McCaffrey, Kimberly E (16)
|
MSMMST |
57.06 |
58.42 |
1.36 |
| 5 |
Richbourg, Caroline K (18)
|
NTMTRO |
57.03 |
58.67 |
1.64 |
| 6 |
Greer, Rani L (17)
|
MSSWAT |
59.47 |
59.34 |
-0.13 |
| 7 |
Dawson, Sydney A (17)
|
LACOLA |
59.13 |
59.39 |
0.26 |
| 8 |
Shipp, Aspen T (16)
|
LACOLA |
1:00.57 |
59.46 |
-1.11 |
| 9 |
Thomas, Ella C (15)
|
MSMMST |
1:00.68 |
59.67 |
-1.01 |
| 10 |
Sammons, Emma L (17)
|
MSMMST |
58.62 |
1:00.02 |
1.40 |
| 11 |
Zhang, Evelyn L (16)
|
LAYNWL |
1:03.25 |
1:00.14 |
-3.11 |
| 12 |
Covington, Sarah H (15)
|
MSMMST |
59.40 |
1:01.04 |
1.64 |
| 13 |
Sammons, Lily K (13)
|
MSMMST |
1:02.28 |
1:02.25 |
-0.03 |
| 14 |
Munson, Caroline E (16)
|
LATAQ |
1:02.43 |
1:02.64 |
0.21 |
| 15 |
Drozdz, Julia B (14)
|
LAYNWL |
1:02.00 |
1:02.85 |
0.85 |
| 16 |
Munger, Julia M (17)
|
LAWAVE |
1:01.78 |
1:03.21 |
1.43 |
| 17 |
Boudreaux, Claire E (16)
|
LACOLA |
1:07.79 |
1:03.52 |
-4.27 |
| 18 |
Bogue, Caroline E (17)
|
NTTRAC |
1:02.26 |
1:04.62 |
2.36 |
| 19 |
Roper, Claire M (13)
|
LAYNWL |
1:05.97 |
1:04.77 |
-1.20 |
| 20 |
Delgado, Liliana N (16)
|
LACOLA |
|
1:05.01 |
- |
| 21 |
Grenrood, Ellen D (13)
|
LACREW |
1:06.21 |
1:05.40 |
-0.81 |