| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mizuba, Hannah R (12)
|
VASFY |
30.03 |
29.93 |
-0.10 |
| 2 |
Fenton, Frances E (12)
|
VALY |
30.46 |
30.02 |
-0.44 |
| 3 |
Wenert, Olivia G (11)
|
VACYAC |
32.87 |
31.89 |
-0.98 |
| 4 |
Phillips, Lillian E (11)
|
VACYAC |
33.78 |
33.35 |
-0.43 |
| 5 |
Muminovic, Anya S (11)
|
VALY |
34.08 |
33.96 |
-0.12 |
| 6 |
DeSantana-Guidry, Khloe (12)
|
VACYAC |
36.12 |
34.67 |
-1.45 |
| 7 |
Kim, Hannah (11)
|
VALY |
40.69 |
35.42 |
-5.27 |
| 8 |
Judy, Olivia C (11)
|
VALY |
37.17 |
36.68 |
-0.49 |
| 9 |
Kovarik, Katherine A (12)
|
VALY |
38.45 |
36.72 |
-1.73 |
| 10 |
Daly, Norah G (12)
|
VALY |
|
38.74 |
- |
| 11 |
Younger, Jordyn I (12)
|
VASFY |
39.67 |
38.75 |
-0.92 |
| 12 |
Fournier, Anne D (12)
|
VALY |
44.23 |
40.82 |
-3.41 |
| 13 |
Koscielny, Eloise C (11)
|
VALY |
55.74 |
50.37 |
-5.37 |
| 14 |
Kirby, Ava G (12)
|
VALY |
49.32 |
51.78 |
2.46 |
|
Taylor, Anna G (11)
|
VALY |
48.39 |
NS |
- |