| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Casey, Nora K (11)
|
MAFWAC |
44.50 |
40.17 |
-4.33 |
| 2 |
Mason, Charlotte A (11)
|
MABRYU |
43.00 |
41.14 |
-1.86 |
| 3 |
Smith, Marielle C (11)
|
MAMSA |
43.34 |
42.87 |
-0.47 |
| 4 |
Zabiegala, Veronica (11)
|
MAUN-0 |
45.35 |
43.39 |
-1.96 |
| 5 |
D'Alesio, Jenna (11)
|
MACAA |
44.43 |
43.53 |
-0.90 |
| 6 |
Lewis, Ryann R (11)
|
MAVINY |
45.94 |
43.61 |
-2.33 |
| 7 |
Agnello, Audrey G (11)
|
MAUN-0 |
47.20 |
44.17 |
-3.03 |
| 7 |
Ng, Milani L (11)
|
MAWAC |
43.24 |
44.17 |
0.93 |
| 9 |
Caraballo, Natalia R (11)
|
MAWAC |
44.76 |
44.56 |
-0.20 |
| 10 |
Geiger, Reese R (11)
|
MAMSC |
43.71 |
44.59 |
0.88 |
| 11 |
Berkstresser, Hannah K (11)
|
MACYA |
46.16 |
44.69 |
-1.47 |
| 12 |
Wagner, Emily R (11)
|
MAJW |
46.05 |
45.42 |
-0.63 |
| 13 |
Bourassa, Ryleigh J (11)
|
MACYA |
44.88 |
45.87 |
0.99 |
| 14 |
Belair, Lilly J (11)
|
MAWAC |
49.92 |
46.05 |
-3.87 |
| 15 |
Drajem, Allie (11)
|
MAWASC |
50.00 |
46.94 |
-3.06 |
| 16 |
Rushdy, Hanna M (11)
|
MAJW |
47.22 |
47.47 |
0.25 |
| 17 |
McCaskill, Aila T (11)
|
MABRY |
46.40 |
47.57 |
1.17 |
| 18 |
Marks, Hannah G (11)
|
MAMSC |
47.44 |
47.81 |
0.37 |
| 19 |
Roselli, Julia M (11)
|
MAACAC |
52.67 |
48.26 |
-4.41 |