| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Calderon, Lucas S (17)
|
MDNBAC |
46.43 |
47.38 |
0.95 |
| 2 |
Hurley, Thomas J (18)
|
MDNBAC |
46.54 |
47.68 |
1.14 |
| 3 |
Hoogenboom, William H (18)
|
MDNBAC |
45.03 |
47.84 |
2.81 |
| 4 |
Merani, Ryan B (15)
|
MDNBAC |
47.19 |
48.52 |
1.33 |
| 5 |
Subong, Nicholas P (16)
|
MDNBAC |
49.62 |
49.21 |
-0.41 |
| 6 |
Sorensen, Alex J (18)
|
MDNBAC |
49.36 |
51.03 |
1.67 |
| 7 |
Wang, Ethan F (15)
|
MAYY |
50.04 |
51.10 |
1.06 |
| 8 |
Cox, Miles C (18)
|
MAYY |
49.17 |
51.17 |
2.00 |
| 9 |
Arsenault, Evan G (15)
|
MDNBAC |
51.59 |
51.49 |
-0.10 |
| 9 |
Gordon, Nicholas M (17)
|
MDNBAC |
48.83 |
51.49 |
2.66 |
| 11 |
Ventura, Timmy J (16)
|
MAYY |
55.90 |
53.81 |
-2.09 |
| 12 |
Assoufid, Alexander N (16)
|
MDNBAC |
53.75 |
54.41 |
0.66 |
| 13 |
Milman, Sean (15)
|
MDNBAC |
55.00 |
55.35 |
0.35 |
| 14 |
Driscoll, Moseley (15)
|
MAYY |
55.13 |
55.40 |
0.27 |