| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tomlinson, Alyssa R (12)
|
NTMAC |
37.22 |
35.26 |
-1.96 |
| 2 |
Younger, Summer N (12)
|
NTMAC |
38.55 |
37.10 |
-1.45 |
| 3 |
Giesinger, Georgia G (11)
|
NTLTSD |
41.31 |
39.77 |
-1.54 |
| 4 |
Stewart, Sabrina G (12)
|
NTMTRO |
39.98 |
39.88 |
-0.10 |
| 5 |
O'Connell, Ryan A (11)
|
NTMAC |
40.62 |
40.85 |
0.23 |
| 6 |
Satterfield, Layna M (11)
|
NTMTRO |
39.88 |
40.95 |
1.07 |
| 7 |
Bane, Sophie M (11)
|
NTMAC |
41.52 |
41.76 |
0.24 |
| 8 |
Costa, Angelia E (11)
|
NTMAC |
44.36 |
42.99 |
-1.37 |
| 9 |
Ewert, Charlotte G (11)
|
NTLTSD |
49.70 |
43.91 |
-5.79 |
| 10 |
Irizarry-Loiz, Kamila L (12)
|
NTMTRO |
45.89 |
48.35 |
2.46 |
| 11 |
Wright, Dahlia R (11)
|
NTLTSD |
|
49.41 |
- |
|
Cardenas, Natalie N (11)
|
NTLTSD |
|
NS |
- |