| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mitter, Rachel L (13)
|
OHKEY |
2:24.21 |
2:25.67 |
1.46 |
| 2 |
Wentzel, Hannah R (13)
|
OHKEY |
2:32.47 |
2:31.22 |
-1.25 |
| 3 |
Kern, Alyssa G (14)
|
OHMCY |
2:27.54 |
2:32.30 |
4.76 |
| 4 |
Belt, Sarah L (13)
|
OHKEY |
2:32.30 |
2:32.31 |
0.01 |
| 5 |
Bent, Dakota R (14)
|
OHCFY |
2:37.44 |
2:35.89 |
-1.55 |
| 6 |
Cordonnier, Reese M (13)
|
OHUCFY |
2:37.41 |
2:36.88 |
-0.53 |
| 7 |
White, Ella E (13)
|
OHMCY |
2:39.14 |
2:37.92 |
-1.22 |
| 8 |
Overholser, Dakota B (13)
|
OHDCY |
2:24.32 |
2:38.77 |
14.45 |
| 9 |
Poffenberger, Abigayle (13)
|
OHMCY |
|
2:51.70 |
- |
|
Eubank, Madalyn E (13)
|
OHCFY |
2:44.22 |
NS |
- |