| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schwebach, Owen W (13)
|
IADSMY |
23.82 |
23.68 |
-0.14 |
| 2 |
Atkinson, Logan J (14)
|
MNRSC |
24.02 |
24.05 |
0.03 |
| 3 |
Niemiec, David M (14)
|
WILAKE |
23.95 |
24.19 |
0.24 |
| 3 |
Haeuser, Mateo D (13)
|
WILAKE |
25.21 |
24.19 |
-1.02 |
| 5 |
Johnston, Hayden R (13)
|
IARIP |
29.21 |
24.39 |
-4.82 |
| 6 |
Guillen Kenigsberg, Leobardo (13)
|
MNFOXJ |
24.48 |
24.58 |
0.10 |
| 7 |
Hansen, Tanner A (13)
|
WILAKE |
24.52 |
24.76 |
0.24 |
| 8 |
Williams, Luke M (14)
|
MNFOXJ |
25.19 |
25.00 |
-0.19 |
| 9 |
Armour, Jackson G (13)
|
MNAQJT |
29.00 |
25.05 |
-3.95 |
| 10 |
Folks, Charles M (14)
|
MNFOXJ |
24.49 |
25.30 |
0.81 |
| 11 |
Shin, Andrew K (14)
|
MNRSC |
24.94 |
25.36 |
0.42 |
| 12 |
Wei, Arthur (13)
|
MNAQJT |
25.52 |
25.38 |
-0.14 |
| 13 |
Choi, Myonghai E (14)
|
MNMTKA |
25.17 |
25.40 |
0.23 |
| 14 |
Ketcham, Zac M (14)
|
IADSMY |
25.47 |
25.61 |
0.14 |
| 15 |
Johnson, Lucas T (13)
|
MNFOXJ |
27.24 |
28.03 |
0.79 |
| 16 |
Zhou, Calvin (13)
|
MNFOXJ |
28.21 |
28.18 |
-0.03 |
| 17 |
Madireddy, Abhijit Reddy (13)
|
MNFOXJ |
29.48 |
28.69 |
-0.79 |
| 18 |
Raney, Grayden J (13)
|
MNFOXJ |
29.95 |
31.19 |
1.24 |
|
Harshbarger, Andrew W (13)
|
WILAKE |
|
25.37 |
- |
|
Rosenhagen, Leo F (13)
|
MNAQJT |
|
25.87 |
- |
|
Havermann, Aidynn J (13)
|
MNSEMS |
|
25.90 |
- |
|
Holmes, Tucker A (14)
|
MNRSC |
|
26.08 |
- |
|
Singewald, Caleb A (13)
|
MNFOXJ |
34.73 |
NS |
- |
|
Gilligan, Cole P (13)
|
MNFOXJ |
31.01 |
NS |
- |