| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Flouras, Anastasia (10)
|
MRQNS |
42.12 |
40.03 |
-2.09 |
| 2 |
Bronskikh, Valeriia N (10)
|
MRFREE |
41.07 |
40.31 |
-0.76 |
| 3 |
Spencer-Edwards, Lauren A (10)
|
MRYMID |
43.44 |
41.86 |
-1.58 |
| 4 |
O'Sullivan, Brinan K (10)
|
MRQNS |
46.09 |
47.58 |
1.49 |
| 5 |
Tang, Addison (10)
|
MRQNS |
1:00.00 |
54.76 |
-5.24 |
| 6 |
Li, Lauren M (10)
|
MRFREE |
57.02 |
55.47 |
-1.55 |
| 7 |
McGeary, Chloe L (10)
|
MRFREE |
57.21 |
55.50 |
-1.71 |
| 8 |
Thai, Kallie L (10)
|
MRQNS |
58.37 |
56.83 |
-1.54 |
| 9 |
Sadek, Marina M (10)
|
MRFREE |
59.99 |
1:05.83 |
5.84 |
|
Daniel, Emma (10)
|
MRLIFE |
45.45 |
NS |
- |
|
Daniel, Gabrielle (10)
|
MRLIFE |
49.12 |
NS |
- |