| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chau, Calvin W (14)
|
MRCIY |
26.05 |
26.42 |
0.37 |
| 2 |
Cordova, Christian M (14)
|
MRCIY |
27.54 |
26.94 |
-0.60 |
| 3 |
Chang, Steven R (14)
|
MRFLY |
28.58 |
27.05 |
-1.53 |
| 4 |
Chen, Alvin (14)
|
MRFLY |
28.60 |
27.23 |
-1.37 |
| 5 |
Mountantonakis, Emmanouil S (13)
|
MRFLY |
31.96 |
28.16 |
-3.80 |
| 6 |
Chang, Edward (14)
|
MRFLY |
30.55 |
28.18 |
-2.37 |
| 7 |
Davis-Whittaker, Jose (13)
|
MRFLY |
30.03 |
28.21 |
-1.82 |
| 8 |
Zhao, Colin E (14)
|
MRFLY |
29.79 |
29.03 |
-0.76 |
| 9 |
Fiechter, Michael A (13)
|
MRCIY |
31.48 |
29.24 |
-2.24 |
| 10 |
O'Sullivan, Kealan J (13)
|
MRCIY |
31.61 |
29.56 |
-2.05 |
| 11 |
Squires, Benjamin (13)
|
MRMCBY |
37.09 |
30.01 |
-7.08 |
| 12 |
Bice, Cian G (13)
|
MRCIY |
29.99 |
30.06 |
0.07 |
| 13 |
Mezzoli, Davide (13)
|
MRLGAC |
|
30.26 |
- |
| 14 |
Tsetlin, Max A (13)
|
MRMCBY |
34.51 |
30.61 |
-3.90 |
| 15 |
De Jesus, Eric (13)
|
MRFLY |
|
30.77 |
- |
| 16 |
Villegas, James J (14)
|
MRRED |
35.11 |
34.31 |
-0.80 |
| 17 |
Wynn Tar-Oo, Vincent (13)
|
MRFLY |
|
34.51 |
- |
| 18 |
Cedeno, Evan (14)
|
MRFLY |
|
45.10 |
- |
| 19 |
Abreu, Gabriel D (13)
|
MRMCBY |
|
45.22 |
- |