| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chau, Calvin W (14)
|
MRCIY |
24.39 |
24.48 |
0.09 |
| 2 |
Mountantonakis, Emmanouil S (13)
|
MRFLY |
24.16 |
24.50 |
0.34 |
| 3 |
Chang, Edward (14)
|
MRFLY |
24.41 |
24.63 |
0.22 |
| 4 |
Chang, Steven R (14)
|
MRFLY |
23.89 |
24.97 |
1.08 |
| 5 |
Cordova, Christian M (14)
|
MRCIY |
25.52 |
25.00 |
-0.52 |
| 6 |
Tesovic, Maksim (14)
|
MRLGAC |
24.34 |
25.08 |
0.74 |
| 7 |
Davis-Whittaker, Jose (13)
|
MRFLY |
25.45 |
25.22 |
-0.23 |
| 8 |
Fiechter, Michael A (13)
|
MRCIY |
24.69 |
25.31 |
0.62 |
| 9 |
Mezzoli, Davide (13)
|
MRLGAC |
|
25.33 |
- |
| 10 |
Chen, Alvin (14)
|
MRFLY |
25.29 |
26.22 |
0.93 |
| 11 |
Zhao, Colin E (14)
|
MRFLY |
25.39 |
26.39 |
1.00 |
| 12 |
O'Sullivan, Kealan J (13)
|
MRCIY |
26.27 |
26.44 |
0.17 |
| 13 |
Tsetlin, Max A (13)
|
MRMCBY |
26.85 |
27.03 |
0.18 |
| 14 |
Bice, Cian G (13)
|
MRCIY |
28.11 |
27.98 |
-0.13 |
| 15 |
Villegas, James J (14)
|
MRRED |
27.91 |
28.28 |
0.37 |
| 16 |
Cedeno, Evan (14)
|
MRFLY |
28.51 |
28.42 |
-0.09 |
| 17 |
Wynn Tar-Oo, Vincent (13)
|
MRFLY |
30.07 |
29.04 |
-1.03 |
| 18 |
Alleyne, Jeremy A (14)
|
MRCIY |
36.59 |
29.21 |
-7.38 |
| 19 |
De Jesus, Eric (13)
|
MRFLY |
29.65 |
29.24 |
-0.41 |
| 20 |
Squires, Benjamin (13)
|
MRMCBY |
34.83 |
31.56 |
-3.27 |
| 21 |
Carrington, Zechariah K (13)
|
MRCIY |
34.29 |
32.67 |
-1.62 |
| 22 |
Abreu, Gabriel D (13)
|
MRMCBY |
33.62 |
33.41 |
-0.21 |