| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chau, Calvin W (14)
|
MRCIY |
27.94 |
26.81 |
-1.13 |
| 2 |
Cordova, Christian M (14)
|
MRCIY |
27.49 |
27.30 |
-0.19 |
| 3 |
Mountantonakis, Emmanouil S (13)
|
MRFLY |
27.40 |
27.39 |
-0.01 |
| 4 |
Chang, Steven R (14)
|
MRFLY |
31.57 |
29.46 |
-2.11 |
| 5 |
Chen, Alvin (14)
|
MRFLY |
29.94 |
30.16 |
0.22 |
| 6 |
O'Sullivan, Kealan J (13)
|
MRCIY |
30.95 |
30.41 |
-0.54 |
| 7 |
Fiechter, Michael A (13)
|
MRCIY |
32.91 |
30.66 |
-2.25 |
| 8 |
Chang, Edward (14)
|
MRFLY |
34.49 |
31.24 |
-3.25 |
| 9 |
Bice, Cian G (13)
|
MRCIY |
32.35 |
31.64 |
-0.71 |
| 10 |
Villegas, James J (14)
|
MRRED |
33.53 |
33.34 |
-0.19 |
| 11 |
Davis-Whittaker, Jose (13)
|
MRFLY |
34.59 |
33.43 |
-1.16 |
| 11 |
Mezzoli, Davide (13)
|
MRLGAC |
|
33.43 |
- |
| 13 |
De Jesus, Eric (13)
|
MRFLY |
33.35 |
33.49 |
0.14 |
| 14 |
Zhao, Colin E (14)
|
MRFLY |
33.52 |
34.69 |
1.17 |
| 15 |
Alleyne, Jeremy A (14)
|
MRCIY |
46.71 |
35.76 |
-10.95 |
| 16 |
Cedeno, Evan (14)
|
MRFLY |
44.40 |
36.00 |
-8.40 |
| 17 |
Wynn Tar-Oo, Vincent (13)
|
MRFLY |
|
37.89 |
- |
| 18 |
Carrington, Zechariah K (13)
|
MRCIY |
44.77 |
43.92 |
-0.85 |
|
Abreu, Gabriel D (13)
|
MRMCBY |
|
DQ |
- |