| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Green, Daphne B (7)
|
MRRA |
46.44 |
45.58 |
-0.86 |
| 2 |
Kuzicki, Isabella R (8)
|
MRAGMV |
52.94 |
54.01 |
1.07 |
| 3 |
Joachim, Gianna M (8)
|
MRNBS |
53.67 |
54.53 |
0.86 |
| 4 |
Matyushina, Daria R (7)
|
MRDVWM |
1:03.49 |
58.15 |
-5.34 |
| 5 |
Butler, Mikayla S (8)
|
MRWSSC |
1:15.52 |
1:04.58 |
-10.94 |
| 6 |
Martinez Santos, Meyali (8)
|
MRMHHW |
1:04.44 |
1:04.78 |
0.34 |
| 7 |
Bao, Hanyin (7)
|
MRNYSA |
|
1:09.66 |
- |
| 8 |
Cabrera-Zavala, Allison (7)
|
MRMHHW |
1:09.43 |
1:12.57 |
3.14 |
| 9 |
Kuzicki, Kendra J (7)
|
MRAGMV |
1:12.78 |
1:16.84 |
4.06 |
|
Sarnicki, Brielle A (7)
|
MRAGMV |
1:45.89 |
DQ |
- |
|
Touijar, Zineb (7)
|
MRMHHW |
1:20.35 |
DQ |
- |
|
Carrigan, Kaylee (7)
|
MRRA |
1:15.00 |
DQ |
- |