| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark, Campbell U (13)
|
MRHVD |
4:55.33 |
5:01.09 |
5.76 |
| 2 |
Vos, Brooke C (13)
|
MRRFAC |
4:59.47 |
5:03.31 |
3.84 |
| 3 |
Mahon, Grace N (14)
|
CTARAC |
5:00.36 |
5:07.96 |
7.60 |
| 4 |
Petriccione, Melania C (13)
|
MRRA |
5:07.60 |
5:09.70 |
2.10 |
| 5 |
Ullinger, Hannah M (14)
|
MRHVD |
|
5:10.09 |
- |
| 6 |
Bodie, Elizabeth (13)
|
CTARAC |
5:16.75 |
5:17.54 |
0.79 |
| 7 |
Horgan, Rosemary E (13)
|
MRHVD |
|
5:27.17 |
- |
| 8 |
Ramoino, Alexandra G (14)
|
MRRA |
5:29.06 |
5:40.75 |
11.69 |
| 9 |
Berg, Peyton K (13)
|
NJNJB |
5:29.67 |
5:42.14 |
12.47 |
| 10 |
Donovan, Erin D (14)
|
MRYBAR |
6:00.75 |
5:58.85 |
-1.90 |
| 11 |
Nguyen, Olivia R (14)
|
MRHVD |
|
6:08.43 |
- |
| 12 |
Bhagaloo, Kayla A (14)
|
NJNJB |
6:03.03 |
6:10.87 |
7.84 |
| 13 |
Donovan, Ciara C (14)
|
MRYBAR |
6:16.87 |
6:12.56 |
-4.31 |
| 14 |
McAuley, Nadia A (11)
|
MRAGMV |
6:02.11 |
6:14.24 |
12.13 |