| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Esposito, Grace (10)
|
MRTRI |
36.00 |
35.89 |
-0.11 |
| 2 |
Briceno, Isabella (10)
|
MRNYSA |
35.84 |
36.16 |
0.32 |
| 3 |
Price, Lily K (10)
|
MRTRI |
37.31 |
37.99 |
0.68 |
| 4 |
Tawil, Alexandra C (7)
|
MRTRI |
50.78 |
43.44 |
-7.34 |
| 5 |
Goncharuk, Abigail E (10)
|
MRTRI |
44.81 |
43.81 |
-1.00 |
| 6 |
Grob, Riley C (9)
|
MRNYSA |
45.63 |
43.97 |
-1.66 |
| 7 |
Arevalo, Emma E (9)
|
MRNYSA |
50.24 |
45.01 |
-5.23 |
| 8 |
Sirkowa, Adelina C (10)
|
MRNYSA |
45.93 |
45.23 |
-0.70 |
| 9 |
Tawil, Ella C (10)
|
MRTRI |
50.19 |
45.90 |
-4.29 |
| 10 |
Van Name, Lily (10)
|
MRTRI |
45.54 |
46.66 |
1.12 |
| 11 |
Zellner-Portela, Natasha N (10)
|
MRNYSA |
53.08 |
55.30 |
2.22 |
|
Costello, Kathryn (9)
|
MRTRI |
52.82 |
NS |
- |