| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Jovie M (12)
|
NCCVAC |
41.79 |
39.55 |
-2.24 |
| 2 |
Terhardt, Kayla A (12)
|
NCRCST |
42.36 |
40.74 |
-1.62 |
| 3 |
Alvarez, Dayana N (12)
|
NCCVAC |
43.89 |
42.08 |
-1.81 |
| 4 |
Robinson, Lillian B (11)
|
NCGG |
42.34 |
42.76 |
0.42 |
| 5 |
Jones, Reilly E (12)
|
NCWA |
44.30 |
42.80 |
-1.50 |
| 6 |
Seraz, Larissa F (12)
|
NCCVAC |
47.02 |
42.91 |
-4.11 |
| 7 |
Sims, Ada T (11)
|
NCRCST |
47.68 |
44.26 |
-3.42 |
| 8 |
Finney, Merrill P (12)
|
NCCVAC |
46.17 |
44.62 |
-1.55 |
| 9 |
Pfeifer, Emma E (11)
|
NCCVAC |
46.95 |
46.63 |
-0.32 |
| 10 |
Pintuff, Jenna R (11)
|
NCRCST |
54.31 |
53.87 |
-0.44 |
|
Pavlinsky, Adaline E (12)
|
NCCVAC |
|
DQ |
- |
|
Mendoza Perez, Erica E (12)
|
NCMSC |
|
DQ |
- |