| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Jovie M (12)
|
NCCVAC |
36.81 |
35.82 |
-0.99 |
| 2 |
Seraz, Larissa F (12)
|
NCCVAC |
43.20 |
38.11 |
-5.09 |
| 3 |
Terhardt, Kayla A (12)
|
NCRCST |
41.89 |
39.60 |
-2.29 |
| 4 |
Alvarez, Dayana N (12)
|
NCCVAC |
41.47 |
40.96 |
-0.51 |
| 5 |
Sims, Ada T (11)
|
NCRCST |
41.16 |
42.13 |
0.97 |
| 6 |
Pfeifer, Emma E (11)
|
NCCVAC |
40.56 |
43.03 |
2.47 |
| 7 |
Robinson, Lillian B (11)
|
NCGG |
41.69 |
43.24 |
1.55 |
| 8 |
Finney, Merrill P (12)
|
NCCVAC |
48.31 |
43.80 |
-4.51 |
| 9 |
Pavlinsky, Adaline E (12)
|
NCCVAC |
|
47.05 |
- |
| 10 |
Pintuff, Jenna R (11)
|
NCRCST |
46.80 |
47.25 |
0.45 |
| 12 |
Short, Addison L (11)
|
NCRCST |
59.46 |
54.84 |
-4.62 |
| 13 |
Casiano, Paula (12)
|
NCCVAC |
|
55.74 |
- |
| 14 |
Roper, Emma K (11)
|
NCMSC |
|
1:23.24 |
- |
| 15 |
Mendoza Perez, Erica E (12)
|
NCMSC |
|
1:24.50 |
- |