| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hawthorne, Maxwell S (12)
|
NCWOW |
32.32 |
30.42 |
-1.90 |
| 2 |
House, William E (12)
|
NCWOW |
32.94 |
31.38 |
-1.56 |
| 3 |
Andrews, Brycen M (11)
|
NCECA |
36.33 |
35.01 |
-1.32 |
| 4 |
Jones, Turner D (12)
|
NCECA |
|
35.08 |
- |
| 5 |
Harris, Joshua B (11)
|
NCWOW |
38.20 |
35.81 |
-2.39 |
| 6 |
Johnson, Samuel T (12)
|
NCECA |
38.62 |
36.90 |
-1.72 |
| 7 |
Butner, Stephen A (11)
|
NCWOW |
34.51 |
37.09 |
2.58 |
| 8 |
Blair, Wesley O (11)
|
NCECA |
40.06 |
39.19 |
-0.87 |
| 9 |
Bullard, Cameron E (12)
|
NCECA |
36.33 |
39.30 |
2.97 |
| 10 |
Hastings, Brady T (11)
|
NCECA |
43.03 |
40.94 |
-2.09 |
| 11 |
Youngblood, Braeden C (11)
|
NCECA |
45.08 |
41.61 |
-3.47 |
| 12 |
Simo, Parker M (11)
|
NCECA |
45.53 |
42.02 |
-3.51 |
| 13 |
Moore, Landon J (12)
|
NCECA |
41.92 |
43.55 |
1.63 |
| 14 |
Carman, Eric M (11)
|
NCECA |
53.55 |
48.22 |
-5.33 |
| 15 |
Sanborn, Noah B (11)
|
NCECA |
|
49.60 |
- |
| 16 |
Lichtenstein, Aiden R (11)
|
NCECA |
|
57.65 |
- |