| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ritchey, Scarlett E (6)
|
NCCAT |
38.24 |
25.81 |
-12.43 |
| 2 |
Sadgrove, Irene J (6)
|
NCCAT |
34.56 |
29.02 |
-5.54 |
| 3 |
Moccari, Leah A (6)
|
NCRSA |
30.07 |
29.08 |
-0.99 |
| 4 |
Hauser, Audrey L (6)
|
NCGFSC |
|
30.52 |
- |
| 5 |
Steinbeck, Rosemary C (6)
|
NCYOTA |
30.32 |
32.02 |
1.70 |
| 6 |
Green, Cadence A (6)
|
NCYOTA |
34.76 |
33.35 |
-1.41 |
| 7 |
Yorkery, Skylar M (6)
|
NCCAT |
45.67 |
33.46 |
-12.21 |
| 8 |
Camerino, Milana E (6)
|
NCRSA |
43.25 |
37.32 |
-5.93 |
| 9 |
Leute, Everly R (6)
|
NCGFSC |
34.74 |
37.51 |
2.77 |
|
Kiefer, Londyn A (6)
|
NCMOR |
37.71 |
DQ |
- |
|
King, Molly F (6)
|
NCMOR |
39.80 |
DQ |
- |
|
Bakelaar, Elizabeth S (6)
|
NCYOTA |
32.32 |
DQ |
- |
|
Swannack, Rose E (6)
|
NCYOTA |
|
DQ |
- |
|
Young, Anabelle M (6)
|
NCYOTA |
|
DQ |
- |