| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
West, Carson J (11)
|
NCMAC |
42.22 |
40.77 |
-1.45 |
| 2 |
Kelley, Keegan M (11)
|
NCMSA |
41.36 |
41.01 |
-0.35 |
| 3 |
Sutorus, Charles R (11)
|
NCMAC |
43.28 |
42.07 |
-1.21 |
| 4 |
Evans, Mitchell S (11)
|
NCMSA |
43.13 |
42.47 |
-0.66 |
| 5 |
Myers, Evan J (11)
|
NCMAC |
42.64 |
42.56 |
-0.08 |
| 6 |
Swett, Matthew P (11)
|
NCTEAM |
42.45 |
43.06 |
0.61 |
| 7 |
Williams, Collin B (11)
|
NCMSA |
43.55 |
43.62 |
0.07 |
| 8 |
Haines, Gavin M (11)
|
NCMSA |
44.23 |
43.64 |
-0.59 |
| 9 |
Dheel, Gage E (11)
|
NCMAC |
45.00 |
44.18 |
-0.82 |
| 10 |
Klepar, Joseph G (11)
|
NCMSA |
44.41 |
44.46 |
0.05 |
| 11 |
Kieffer, Thomas J (11)
|
NCMAC |
44.38 |
44.50 |
0.12 |
| 12 |
Stewart, Dylan P (11)
|
NCMSA |
45.91 |
44.65 |
-1.26 |
| 13 |
Mark, Ayden B (11)
|
NCMSA |
43.13 |
44.66 |
1.53 |
| 14 |
Speckman, Brody H (11)
|
NCMAC |
45.92 |
45.98 |
0.06 |
| 15 |
Austin, Elliott B (11)
|
NCMSA |
45.82 |
47.65 |
1.83 |
| 16 |
Stroupe, Charles B (11)
|
NCMSA |
52.78 |
47.68 |
-5.10 |
| 17 |
McBryde, Colton L (11)
|
NCMSA |
47.85 |
47.82 |
-0.03 |
| 18 |
Wood, Cameron W (11)
|
NCMSA |
47.88 |
47.95 |
0.07 |
| 19 |
Weinberg, Evan G (11)
|
NCTEAM |
45.99 |
48.18 |
2.19 |
| 20 |
Shukhnov, Phillip A (11)
|
NCMAC |
47.78 |
48.26 |
0.48 |
| 21 |
Karlsson, Jakob T (11)
|
NCNSS |
50.46 |
48.39 |
-2.07 |
| 22 |
Mason, Evan T (11)
|
NCMAC |
47.57 |
49.33 |
1.76 |
| 23 |
Nettles, Grady S (11)
|
NCMSA |
57.39 |
50.57 |
-6.82 |
| 24 |
McBurney, Maxwell R (11)
|
NCMSA |
50.41 |
50.58 |
0.17 |
| 25 |
Lanoue, Drew B (11)
|
NCTEAM |
50.99 |
51.03 |
0.04 |
| 26 |
Dave, Arya (11)
|
NCMSA |
51.83 |
51.49 |
-0.34 |
| 27 |
Smith, Charles K (11)
|
NCMAC |
53.45 |
52.39 |
-1.06 |
| 28 |
Haggerty, James H (11)
|
NCMSA |
54.57 |
52.94 |
-1.63 |
| 29 |
Lawrie, Silas M (11)
|
NCNSS |
54.41 |
53.05 |
-1.36 |
| 30 |
Michel, Ethan S (11)
|
NCMSA |
56.51 |
54.68 |
-1.83 |
| 31 |
Wolf, Truett (11)
|
NCMAC |
|
55.23 |
- |
| 32 |
Leonard, Zackary H (11)
|
NCMAC |
55.84 |
55.30 |
-0.54 |
| 33 |
Oconnor, Christopher G (11)
|
NCMAC |
57.28 |
56.25 |
-1.03 |
| 34 |
Clair, Cooper P (11)
|
NCMAC |
57.04 |
1:00.50 |
3.46 |
| 35 |
Kosse, Owen T (11)
|
NCMAC |
56.69 |
1:00.90 |
4.21 |
| 36 |
Ala, Anirudh (11)
|
NCMSA |
58.67 |
1:01.16 |
2.49 |
| 37 |
Jones, Sean M (11)
|
NCMAC |
1:08.18 |
1:01.41 |
-6.77 |
| 38 |
Hite, Aidan C (11)
|
NCMAC |
1:01.12 |
1:01.81 |
0.69 |
| 39 |
Barton, James T (11)
|
NCMSA |
1:00.00 |
1:07.00 |
7.00 |
|
Lumbanbatu Solano, Andres S (11)
|
NCMAC |
57.11 |
DQ |
- |
|
Fortier, Seth C (11)
|
NCMSA |
|
DQ |
- |