| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Parrott, Grant M (8)
|
NTNTN |
39.12 |
41.66 |
2.54 |
| 2 |
Pepler, Christopher J (8)
|
NTJMSS |
44.21 |
43.70 |
-0.51 |
| 3 |
Case, James S (8)
|
NTESP |
47.27 |
43.96 |
-3.31 |
| 4 |
Seifert, Jacob Z (8)
|
NTNTN |
48.10 |
44.12 |
-3.98 |
| 5 |
McWhorter, Marshall J (8)
|
NTUN |
49.49 |
52.22 |
2.73 |
| 6 |
Pigneri, Michael A (8)
|
NTATAC |
|
53.66 |
- |
| 7 |
Garwood, Benjamin C (8)
|
NTLTSD |
1:14.33 |
57.34 |
-16.99 |
|
Dorsey, Luke H (8)
|
NTATAC |
|
DQ |
- |
|
Li, Hunter (7)
|
NTATAC |
|
DQ |
- |
|
Thabane, Neo J (7)
|
NTLTSD |
|
DQ |
- |
|
Li, Andy Q (8)
|
NTMTRO |
|
DQ |
- |
|
Fox, Dylan A (8)
|
NTESP |
48.29 |
NS |
- |