| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fox, Hannah A (17)
|
NTESP |
54.27 |
58.05 |
3.78 |
| 2 |
Culbertson, Cadence M (15)
|
NTMTRO |
58.19 |
58.09 |
-0.10 |
| 3 |
Costantini, Amelia L (15)
|
NTSTAR |
58.82 |
1:01.61 |
2.79 |
| 4 |
Gadsby, Madison T (17)
|
NTMTRO |
|
1:03.27 |
- |
| 5 |
Lonsford, Lexey J (15)
|
NTMTRO |
1:09.46 |
1:06.47 |
-2.99 |
| 6 |
Zevallos, Ava G (15)
|
NTSTAR |
1:03.17 |
1:06.65 |
3.48 |
| 7 |
Mather, Hannah E (15)
|
NTSTAR |
1:00.05 |
1:06.90 |
6.85 |
| 8 |
Erwin, Kaylee F (15)
|
NTMTRO |
1:04.89 |
1:07.37 |
2.48 |
| 9 |
Stuelpe, Sydney O (15)
|
NTATAC |
1:09.65 |
1:10.13 |
0.48 |
| 10 |
Gnidziejko, Emma L (16)
|
NTMTRO |
1:00.20 |
1:12.31 |
12.11 |
| 11 |
Perez, Julia G (16)
|
NTSTAR |
1:13.18 |
1:14.26 |
1.08 |
| 12 |
Laxson, Emma T (17)
|
NTMTRO |
|
1:18.48 |
- |
|
Henson, Grace C (16)
|
NTATAC |
1:17.56 |
NS |
- |
|
Axton, Audrey E (15)
|
NTNTN |
1:00.76 |
NS |
- |
|
McAdams, Abigail F (15)
|
NTNTN |
56.07 |
NS |
- |