| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eden, Jessica M (16)
|
GMH |
58.12 |
56.97 |
-1.15 |
| 2 |
MacPhail, Elizabeth G (16)
|
SHHB |
59.66 |
57.87 |
-1.79 |
| 3 |
Sylvan, Lena D (17)
|
GMH |
1:00.55 |
58.14 |
-2.41 |
| 4 |
Doorley, Ashlyn D (15)
|
GMH |
1:01.69 |
58.95 |
-2.74 |
| 5 |
Machado, Madison (14)
|
CFA |
1:03.25 |
59.03 |
-4.22 |
| 6 |
Montisano, Maria R (16)
|
COP |
1:01.00 |
59.78 |
-1.22 |
| 7 |
Katz, Chloe T (16)
|
GMH |
1:02.47 |
1:00.95 |
-1.52 |
| 8 |
Hollington, Katie (17)
|
PPO |
1:02.73 |
1:01.17 |
-1.56 |
| 9 |
Caye, Michelle C (17)
|
COP |
1:02.83 |
1:01.20 |
-1.63 |
| 10 |
Kitchen, Hope (15)
|
PER |
1:02.09 |
1:01.59 |
-0.50 |
| 11 |
Kurtzweil, Bridget J (15)
|
ECAT |
1:03.68 |
1:02.83 |
-0.85 |
| 12 |
Grosenbaugh, Taylor E (18)
|
COP |
1:04.72 |
1:03.23 |
-1.49 |
| 13 |
Smith, Madison P (16)
|
LOU |
1:02.43 |
1:03.42 |
0.99 |
| 14 |
Wellman, Evelyn R (14)
|
SHHB |
1:03.79 |
1:03.48 |
-0.31 |
| 15 |
Klinginsmith, Anna (16)
|
PPO |
1:03.55 |
1:03.85 |
0.30 |
| 16 |
Szuhay, Sierra (17)
|
JAR |
1:06.37 |
1:04.27 |
-2.10 |
| 17 |
Klug, Isabella (17)
|
CFA |
1:07.48 |
1:04.78 |
-2.70 |
| 18 |
Haywood, Daelynn A (14)
|
SHHB |
1:05.79 |
1:04.96 |
-0.83 |
| 19 |
Glime, Mandy (0)
|
CFK |
1:04.78 |
1:05.50 |
0.72 |
| 20 |
Hanek, Izabella (14)
|
JAR |
1:02.32 |
1:05.93 |
3.61 |
| 21 |
Trem, Sophia (14)
|
MLC |
1:07.73 |
1:05.96 |
-1.77 |
| 22 |
Bennett, Maddy (16)
|
NLSR |
1:06.45 |
1:06.39 |
-0.06 |
| 23 |
Bokan, Brooklyn (16)
|
MCD |
1:04.89 |
1:06.47 |
1.58 |
| 24 |
Bochenek, Molly (0)
|
CFK |
1:07.49 |
1:06.65 |
-0.84 |
| 25 |
Zahand, Olivia (0)
|
CLCH |
1:07.37 |
1:07.27 |
-0.10 |
| 26 |
Svette, Grace (14)
|
CFA |
1:07.07 |
1:07.89 |
0.82 |
| 27 |
Montgomery, Megan (14)
|
AMA |
1:05.95 |
1:08.54 |
2.59 |
| 28 |
Meng, Nova (17)
|
RRE |
1:07.69 |
1:09.82 |
2.13 |
|
Bowers, Hannah (16)
|
SAL |
1:04.59 |
DQ |
- |