| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peyton, Ethan A (10)
|
OHDR |
33.96 |
34.64 |
0.68 |
| 2 |
Simon, Elliot X (10)
|
OHKEY |
36.07 |
35.31 |
-0.76 |
| 3 |
Broxterman, Samuel D (10)
|
OHRAYS |
37.28 |
35.40 |
-1.88 |
| 4 |
Schlagetter, Brodie R (10)
|
OHBAC |
39.36 |
35.70 |
-3.66 |
| 5 |
Melton, Ross J (10)
|
OHDR |
38.07 |
37.41 |
-0.66 |
| 6 |
Vamadeva, Velan (10)
|
OHRAYS |
41.42 |
40.25 |
-1.17 |
| 7 |
Howbert, Jack A (10)
|
OHKEY |
45.55 |
43.06 |
-2.49 |
| 8 |
Pathak, Tavish (10)
|
OHRAYS |
42.61 |
44.08 |
1.47 |
| 9 |
Vamadeva, Lingan (10)
|
OHRAYS |
43.95 |
44.21 |
0.26 |
| 10 |
Couvutsakis, Bryce (10)
|
OHPCYP |
47.10 |
45.41 |
-1.69 |
| 11 |
Vawter, Elijah M (10)
|
OHWTRC |
44.62 |
47.06 |
2.44 |
| 12 |
Wang, Liheng J (10)
|
OHLIFE |
56.94 |
49.08 |
-7.86 |
| 13 |
Brazelton, Skyler J (10)
|
OHRAYS |
58.13 |
53.65 |
-4.48 |
| 14 |
Alyanak, Edward J (10)
|
OHKEY |
55.72 |
58.05 |
2.33 |
|
McMullen, Max H (10)
|
OHKEY |
|
DQ |
- |
|
Goel, Shobhit (10)
|
OHWTRC |
|
DQ |
- |
|
Goettke, Joseph E (10)
|
OHWTRC |
59.70 |
DQ |
- |
|
Triola, Dominic A (10)
|
OHWTRC |
1:11.70 |
DQ |
- |
|
Mollet, Maddox A (10)
|
OHLIFE |
|
NS |
- |