| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Talley, Brooklyn M (8)
|
OHDR |
36.12 |
36.24 |
0.12 |
| 2 |
Molina, Julia M (8)
|
OHLAKY |
38.44 |
38.04 |
-0.40 |
| 3 |
Montgomery, Madeline E (8)
|
OHPCYP |
53.27 |
44.02 |
-9.25 |
| 4 |
Heppner, Jacqueline E (8)
|
OHDR |
56.13 |
49.00 |
-7.13 |
| 5 |
Popp, Genevieve G (8)
|
OHWTRC |
1:07.51 |
50.33 |
-17.18 |
| 6 |
Parks, Abigail M (8)
|
OHLAKY |
1:06.72 |
58.32 |
-8.40 |
| 7 |
Vedantam, Miri R (8)
|
OHRAYS |
57.81 |
58.50 |
0.69 |
| 8 |
Brown, Brianna E (8)
|
OHPCYP |
1:03.87 |
58.74 |
-5.13 |
| 9 |
Davis, Shealyn N (8)
|
OHDR |
1:07.31 |
58.90 |
-8.41 |
| 10 |
Loree, Katherine A (8)
|
OHWTRC |
1:02.08 |
1:05.41 |
3.33 |
| 11 |
Pohlmeyer, Kaia M (8)
|
OHRAYS |
1:09.16 |
1:06.37 |
-2.79 |
| 12 |
Sampath, Maithri M (8)
|
OHRAYS |
|
1:10.23 |
- |
|
Watkins, Emilia J (8)
|
OHDR |
|
DQ |
- |
|
Rhea, Katherine E (8)
|
OHLIFE |
|
DQ |
- |
|
Gaikwad, Anaisha (8)
|
OHRAYS |
|
NS |
- |