| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trumpower, Adayaleise L (7)
|
OHUN |
21.05 |
20.77 |
-0.28 |
| 2 |
Stelitano, Mia S (8)
|
AMKW |
25.07 |
23.94 |
-1.13 |
| 3 |
Stannert, Lillian G (8)
|
OHFMST |
25.65 |
25.18 |
-0.47 |
| 4 |
Thomas, Lucy J (8)
|
OHUN |
27.64 |
25.88 |
-1.76 |
| 5 |
Burkey, Brynn M (8)
|
OHFMST |
27.53 |
26.00 |
-1.53 |
| 6 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
27.31 |
26.09 |
-1.22 |
| 7 |
Kin, Emily L (7)
|
OHFAST |
26.91 |
26.51 |
-0.40 |
| 8 |
Laman, Elizabeth P (8)
|
AMKW |
29.02 |
26.64 |
-2.38 |
| 9 |
Rhoades, Emilia M (8)
|
OHSOS |
25.23 |
27.58 |
2.35 |
| 10 |
Wittmer, Marin A (7)
|
OHFMST |
28.45 |
27.62 |
-0.83 |
| 11 |
Clifford, Hazel A (8)
|
AMKW |
33.14 |
28.11 |
-5.03 |
| 12 |
Ukrayinets, Sofiya (8)
|
AMANSC |
33.38 |
28.14 |
-5.24 |
| 13 |
Rando, Maylie P (8)
|
OHSOS |
38.32 |
30.16 |
-8.16 |
| 14 |
Ukrayinets, Anastasiya (8)
|
AMANSC |
35.00 |
31.80 |
-3.20 |
| 15 |
Maddox, Reagan J (8)
|
AMANSC |
35.45 |
32.01 |
-3.44 |
| 16 |
Lopez, Jennifer * (8)
|
OHBGSC |
38.83 |
32.17 |
-6.66 |
| 17 |
Sunday, Alaina J (7)
|
OHFMST |
34.67 |
33.50 |
-1.17 |
| 18 |
Coffman, Margaret J (7)
|
AMANSC |
38.28 |
36.45 |
-1.83 |
| 19 |
Lim, Sharon Y (7)
|
OHSYL |
43.53 |
39.40 |
-4.13 |
| 20 |
Glass, Callie E (6)
|
AMANSC |
40.21 |
42.29 |
2.08 |
|
Hatt, Chloe K (7)
|
LEPCP |
26.90 |
DQ |
- |
|
Starz, Rian O (8)
|
AMKW |
24.02 |
NS |
- |
|
O'Toole, Delaney S (8)
|
AMKW |
19.17 |
NS |
- |
|
Alt, Eden E (8)
|
OHBGSC |
|
NS |
- |