| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simmons, Victoria A (10)
|
PVOCCS |
35.95 |
33.04 |
-2.91 |
| 2 |
Edwards, Amelia L (10)
|
PVNCAP |
37.38 |
34.16 |
-3.22 |
| 3 |
Velarde Maynard, Matilda C (10)
|
PVOCCS |
37.55 |
35.57 |
-1.98 |
| 4 |
Rahmouni, Noel Joy (10)
|
PVNCAP |
36.88 |
35.60 |
-1.28 |
| 5 |
Mount, Alexis J (10)
|
PVNCAP |
1:00.00 |
52.16 |
-7.84 |
| 6 |
Kim, Leah Y (10)
|
PVMAC |
|
52.60 |
- |
| 7 |
Correll, Hannah E (10)
|
PVOCCS |
52.83 |
55.20 |
2.37 |
| 8 |
Fincham, Paige C (10)
|
PVNCAP |
56.92 |
58.48 |
1.56 |
| 9 |
Lugiai, McKenna G (10)
|
PVOCCS |
52.97 |
59.26 |
6.29 |
| 10 |
Stanley, Brooklyn M (10)
|
PVOCCS |
59.91 |
1:03.85 |
3.94 |
| 11 |
Manning, Katherine D (10)
|
PVNCAP |
1:00.00 |
1:04.23 |
4.23 |