| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Iaci, Luca M (12)
|
PVMAKO |
35.16 |
35.98 |
0.82 |
| 2 |
Boyd, Kairo-Aicha F (12)
|
PVSTJS |
39.30 |
37.18 |
-2.12 |
| 3 |
Stevens, Audrey V (11)
|
PVSTJS |
|
37.25 |
- |
| 4 |
Dierks, Anna E (11)
|
PVSTJS |
|
37.29 |
- |
| 5 |
Strnad, Thomas M (11)
|
PVMAKO |
39.42 |
37.88 |
-1.54 |
| 6 |
Montgomery, Nicholas S (11)
|
PVSTJS |
41.87 |
39.42 |
-2.45 |
| 7 |
Murphy, Caitlyn G (11)
|
PVMAKO |
41.40 |
40.43 |
-0.97 |
| 8 |
Scherber, Naomi C (11)
|
PVMAKO |
41.75 |
40.53 |
-1.22 |
| 9 |
Gallacher, Mary E (11)
|
PVSTJS |
56.78 |
47.66 |
-9.12 |
| 10 |
MacRae, Dianna S (11)
|
PVSTJS |
|
48.01 |
- |
| 11 |
Nurgaliyev, Dimash Ali A (12)
|
PVSTJS |
|
48.71 |
- |
|
Brennan, Beckett P (11)
|
PVSTJS |
41.85 |
DQ |
- |
|
Cloutier, Alan K (12)
|
PVMAKO |
|
NS |
- |
|
van de Graaff, Anthony D (11)
|
PVMAKO |
33.73 |
NS |
- |