| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wu, Gabriel B (9)
|
PVMACH |
42.10 |
39.49 |
-2.61 |
| 2 |
Buczek, Anabel H (9)
|
PVMACH |
43.04 |
40.96 |
-2.08 |
| 3 |
Radio, Lucy A (9)
|
PVMACH |
48.81 |
41.76 |
-7.05 |
| 4 |
Yu, Felix P (9)
|
PVMACH |
|
41.88 |
- |
| 5 |
Rivera-Castro, Noelle I (9)
|
PVMACH |
44.80 |
42.64 |
-2.16 |
| 6 |
Pezzimenti, Natalia M (9)
|
PVMACH |
48.79 |
44.69 |
-4.10 |
| 7 |
Tan, Ava E (9)
|
PVMACH |
50.32 |
44.70 |
-5.62 |
| 8 |
Lasut, Claire I (9)
|
PVMACH |
46.69 |
45.15 |
-1.54 |
| 9 |
Roberts Nijim, Sami J (9)
|
PVMACH |
47.94 |
45.39 |
-2.55 |
| 10 |
Cherryholmes, Caleb J (9)
|
PVMACH |
50.38 |
45.73 |
-4.65 |
| 11 |
Showalter, Elle (9)
|
PVMACH |
|
45.94 |
- |
| 12 |
Zhao, Lucas X (9)
|
PVMACH |
48.60 |
46.27 |
-2.33 |
| 13 |
Dalton, Umi J (9)
|
PVMACH |
44.76 |
46.43 |
1.67 |
| 14 |
Pai, Divya I (9)
|
PVMACH |
|
50.06 |
- |
| 15 |
McNamara, Ella G (9)
|
PVMACH |
|
50.36 |
- |
| 16 |
Armanyos, Adam S (9)
|
PVMACH |
|
51.09 |
- |
| 17 |
Engledow, Alice B (9)
|
PVMACH |
49.53 |
51.87 |
2.34 |
| 18 |
Onerci, Gizem (9)
|
PVMACH |
1:01.90 |
57.53 |
-4.37 |
| 19 |
Hejmo, Achilleas K (9)
|
PVMACH |
|
57.76 |
- |
|
Steele, Georgia K (9)
|
PVMACH |
45.90 |
DQ |
- |