| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hull, Christine S (8)
|
PVRED |
21.07 |
20.98 |
-0.09 |
| 2 |
Martinez, Lara A (8)
|
PVGRAY |
23.64 |
22.72 |
-0.92 |
| 3 |
Rossi, Aubrey G (8)
|
PVRED |
21.90 |
24.84 |
2.94 |
| 4 |
Justus, Juliana E (8)
|
PVRED |
22.66 |
25.10 |
2.44 |
| 5 |
Thorpe, Rachel E (8)
|
PVGRAY |
24.26 |
26.07 |
1.81 |
| 6 |
Christensen, Kaitlyn J (8)
|
PVRED |
25.83 |
28.50 |
2.67 |
| 7 |
Zimmer, Aubrey L (8)
|
PVRED |
27.84 |
28.62 |
0.78 |
| 8 |
Hawver, Allison T (8)
|
PVRED |
|
30.56 |
- |
| 9 |
Lugiai, Ainsley R (8)
|
PVGRAY |
29.81 |
31.59 |
1.78 |
| 10 |
Lugiai, Brooke E (8)
|
PVGRAY |
33.20 |
32.62 |
-0.58 |
| 11 |
Frisk, Autumn C (8)
|
PVRED |
|
39.44 |
- |
|
Holmes-Lastovica, Ellie M (8)
|
PVGRAY |
|
DQ |
- |
|
Schoenborn, Zoe E (8)
|
PVGRAY |
|
DQ |
- |
|
Soto, Malaysia R (8)
|
PVRED |
28.38 |
NS |
- |