| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wang, Katherine A (6)
|
PVASA |
25.94 |
24.42 |
-1.52 |
| 2 |
Qaasim, Khayirah (6)
|
PVYASD |
24.27 |
25.11 |
0.84 |
| 3 |
Huang, Yining (6)
|
PVFISH |
23.75 |
25.26 |
1.51 |
| 4 |
Dmitrova, Stephania Z (6)
|
PVFISH |
24.56 |
27.19 |
2.63 |
| 5 |
Francis, Caroline V (6)
|
PVNCAP |
31.85 |
28.00 |
-3.85 |
| 6 |
Shoemaker, Emily M (6)
|
PVNCAP |
25.65 |
28.11 |
2.46 |
| 7 |
Wheatley, Mays D (6)
|
PVFISH |
29.92 |
28.73 |
-1.19 |
| 8 |
Shepard, Rachael N (6)
|
PVFISH |
25.17 |
28.82 |
3.65 |
| 9 |
Wilson-Segovia, Suzannah A (6)
|
PVFBST |
35.96 |
30.85 |
-5.11 |
| 10 |
Hyde, Anna L (6)
|
PVFBST |
32.97 |
31.01 |
-1.96 |