| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kuetemeyer, Gabriella R (15)
|
OHCY |
2:43.79 |
2:37.44 |
-6.35 |
| 2 |
Cunningham, Jacqueline R (16)
|
OHCY |
|
2:38.03 |
- |
| 3 |
Wyers, Alexandria Z (15)
|
OHCFY |
2:45.92 |
2:43.40 |
-2.52 |
| 4 |
Marini, Sofia A (15)
|
OHLAKY |
3:01.10 |
2:45.26 |
-15.84 |
| 5 |
Widenhouse, Delaney S (15)
|
OHCY |
2:46.64 |
2:50.81 |
4.17 |
| 6 |
Jernovics, Emma C (16)
|
OHCFY |
2:44.13 |
2:51.13 |
7.00 |
| 7 |
York, Kristianna E (15)
|
OHCY |
3:04.77 |
2:51.65 |
-13.12 |
| 8 |
Lentine, Lillian C (17)
|
OHCY |
3:11.90 |
2:52.27 |
-19.63 |
| 9 |
Wethington, Faith M (16)
|
OHCY |
2:57.89 |
2:53.82 |
-4.07 |
| 10 |
Smith, Kaitlin (16)
|
OHKEY |
2:55.65 |
2:59.11 |
3.46 |
| 11 |
Jones, Olivia R (17)
|
OHCFY |
2:54.01 |
3:04.16 |
10.15 |