| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kulow, Jonathan P (16)
|
WYLAN |
50.60 |
50.39 |
-0.21 |
| 2 |
Guiliano, Christopher R (17)
|
MATOPS |
50.54 |
50.53 |
-0.01 |
| 3 |
Schiavone, Christopher J (23)
|
MAFNM |
51.01 |
51.47 |
0.46 |
| 4 |
McMahon, Alexander J (18)
|
COAQUA |
|
51.72 |
- |
| 5 |
Lou, Brian (18)
|
CANOVA |
52.40 |
51.74 |
-0.66 |
| 6 |
Sergile, Sebastien X (17)
|
GASA |
51.75 |
51.89 |
0.14 |
| 7 |
Cline, Brendan M (20)
|
MAFNM |
1:07.19 |
52.13 |
-15.06 |
| 8 |
Voss, Avery G (18)
|
OHUASC |
51.37 |
52.20 |
0.83 |
| 9 |
Brown, Lucius Y (17)
|
SICSTE |
52.38 |
52.73 |
0.35 |
| 10 |
Zum Tobel, Maximilian W (17)
|
FGSAS |
51.74 |
52.80 |
1.06 |
| 11 |
Hughes, Parker A (20)
|
AZUN-0 |
51.95 |
53.44 |
1.49 |