| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zuleger, Skylar B (10)
|
NCTYDE |
34.72 |
34.87 |
0.15 |
| 2 |
Herlocker, Patterson L (10)
|
NCRACY |
40.91 |
40.62 |
-0.29 |
| 3 |
Trevino, Gabriella B (9)
|
NCGCY |
41.28 |
40.76 |
-0.52 |
| 4 |
Pace, Clementine S (10)
|
NCTYDE |
41.43 |
40.99 |
-0.44 |
| 5 |
Holmes, Melynn C (10)
|
NCTYDE |
43.33 |
43.16 |
-0.17 |
| 6 |
Bellini, Isabella R (10)
|
NCTYDE |
42.68 |
44.27 |
1.59 |
| 7 |
Smitherman, Katherine M (9)
|
NCTYDE |
43.07 |
44.91 |
1.84 |
| 8 |
Goddard, Kyla J (9)
|
NCGCY |
55.63 |
55.34 |
-0.29 |
| 9 |
Crider, Kendyl A (8)
|
NCTYDE |
|
56.93 |
- |
| 10 |
Robertson, Addilynn G (9)
|
NCTYDE |
|
1:04.06 |
- |
| 11 |
Sechriest, Emory G (9)
|
NCRACY |
1:01.65 |
1:09.40 |
7.75 |
| 12 |
Gentry, Emma C (10)
|
NCLOY |
|
1:12.05 |
- |
|
Wang, Ellen (9)
|
NCGCY |
57.78 |
DQ |
- |
|
Whitworth, Lucy H (9)
|
NCTYDE |
|
DQ |
- |