| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mithqal, Mariam A (14)
|
VACA-Y |
55.94 |
57.14 |
1.20 |
| 2 |
McCardle, Grace H (14)
|
VAACAC |
55.21 |
57.25 |
2.04 |
| 3 |
Schundler, Elizabeth P (14)
|
VACA-Y |
59.15 |
59.58 |
0.43 |
| 4 |
Rogerson, Annika R (13)
|
VAUN- |
1:00.41 |
1:00.64 |
0.23 |
| 5 |
Streit, Molly S (13)
|
VACA-Y |
1:03.19 |
1:01.12 |
-2.07 |
| 6 |
Yost, Bethany O (14)
|
VACA-Y |
1:01.62 |
1:02.96 |
1.34 |
| 7 |
Gahlmann, Kylin E (13)
|
VACA-Y |
1:01.70 |
1:03.06 |
1.36 |
| 8 |
Altherr, Vaile K (14)
|
VAACAC |
1:05.62 |
1:05.33 |
-0.29 |
| 9 |
Stevens, Kirsten M (14)
|
VACA-Y |
1:10.35 |
1:12.22 |
1.87 |
| 10 |
Connaughton, Quinn L (13)
|
VACA-Y |
1:15.76 |
1:17.62 |
1.86 |