| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fuller, Abigail B (17)
|
VAACAC |
52.10 |
52.62 |
0.52 |
| 2 |
Addison, Julia I (15)
|
VACA-Y |
54.62 |
55.87 |
1.25 |
| 3 |
Huang, Avery Z (16)
|
VACA-Y |
53.38 |
56.25 |
2.87 |
| 4 |
Burr, Kathryn A (17)
|
VACA-Y |
52.26 |
57.13 |
4.87 |
| 5 |
Fix, Cayden M (15)
|
VACA-Y |
54.58 |
57.24 |
2.66 |
| 6 |
Sheffield, Haley S (15)
|
VACA-Y |
55.65 |
57.77 |
2.12 |
| 7 |
Yu, Sophia (17)
|
VACA-Y |
53.49 |
58.36 |
4.87 |
| 8 |
Rogerson, Anneliese N (16)
|
VAUN- |
57.61 |
59.11 |
1.50 |
| 9 |
Phillips, Casey R (17)
|
VACA-Y |
58.11 |
59.19 |
1.08 |
| 10 |
Fitzpatrick, Lillian S (17)
|
VACA-Y |
59.98 |
59.85 |
-0.13 |
| 11 |
Shannon, Hannah M (15)
|
VACA-Y |
59.53 |
1:00.43 |
0.90 |
| 12 |
Wells, Madelyn I (16)
|
VACA-Y |
54.47 |
1:00.44 |
5.97 |
| 13 |
Moore, Sarah A (15)
|
VACA-Y |
1:03.77 |
1:03.95 |
0.18 |
|
Haise, Sophie A (17)
|
VAACAC |
54.95 |
NS |
- |
|
Van Beek, Josefine S (15)
|
VACA-Y |
56.27 |
NS |
- |