| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stevens, Jenna L (12)
|
VAHNVR |
39.41 |
38.04 |
-1.37 |
| 2 |
Willard, Katelyn R (11)
|
VAHNVR |
42.10 |
39.83 |
-2.27 |
| 3 |
Lutton, Chloe G (11)
|
VAHNVR |
42.07 |
40.71 |
-1.36 |
| 4 |
Segura, Mara J (11)
|
VATSU |
44.20 |
41.02 |
-3.18 |
| 5 |
Furrow, Susannah C (11)
|
VATSU |
41.80 |
42.15 |
0.35 |
| 6 |
Zyglocke, Lilly M (11)
|
VAHNVR |
|
42.85 |
- |
| 7 |
Duda, Savannah N (12)
|
VAHNVR |
|
42.90 |
- |
| 8 |
Ellington, Juliann R (12)
|
VAHNVR |
|
43.70 |
- |
| 9 |
Beaudoin, Isabella A (12)
|
VAVSTP |
|
47.60 |
- |
| 10 |
Wakefield, Ella G (11)
|
VAHNVR |
50.36 |
50.99 |
0.63 |
| 11 |
Sutton, Kelsey L (11)
|
VAHNVR |
50.35 |
51.20 |
0.85 |
| 12 |
Miller, Sydney L (11)
|
VAHNVR |
53.12 |
52.61 |
-0.51 |