| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schattgen, Reid P (20)
|
VATSU |
49.71 |
50.40 |
0.69 |
| 2 |
Schoenwiesner, Benjamin S (17)
|
VAHNVR |
49.52 |
51.02 |
1.50 |
| 3 |
Warnagiris, Paul D (15)
|
VAWST |
50.91 |
51.93 |
1.02 |
| 4 |
Beaudoin, Benjamin R (16)
|
VAVSTP |
53.54 |
53.26 |
-0.28 |
| 5 |
Cram, Trevor C (17)
|
VAVSTP |
|
53.37 |
- |
| 6 |
Geddes, Owen J (16)
|
VATSU |
52.77 |
53.43 |
0.66 |
| 7 |
Castle, Samuel G (15)
|
VAHNVR |
1:08.14 |
53.44 |
-14.70 |
| 8 |
Post, Aidan J (17)
|
VAVSTP |
54.50 |
54.44 |
-0.06 |
| 9 |
Pruemer, Alexander A (16)
|
VAHNVR |
52.97 |
54.54 |
1.57 |
| 10 |
Fowler, Henry H (16)
|
VAWST |
55.10 |
54.84 |
-0.26 |
| 11 |
Appleton, Matthew R (16)
|
VATSU |
52.77 |
55.90 |
3.13 |
| 12 |
Thompson, Andrew (15)
|
VAWST |
55.16 |
57.11 |
1.95 |
| 13 |
Twum, Zion (16)
|
VATSU |
1:05.60 |
1:02.46 |
-3.14 |
| 14 |
Pollard, Justus C (16)
|
VATSU |
1:09.92 |
1:10.39 |
0.47 |