| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Aguilar, Rody J (21)
|
UNAT |
26.50 |
26.02 |
-0.48 |
| 2 |
Lipka, Sam I (17)
|
NEGS |
27.25 |
27.48 |
0.23 |
| 3 |
Gorsuch, Nathaniel C (14)
|
MEUN |
30.86 |
27.60 |
-3.26 |
| 4 |
Oey, Daevin (16)
|
PVHACC |
34.63 |
28.09 |
-6.54 |
| 5 |
Matter, Taylor O (15)
|
MNMAKO |
32.20 |
28.17 |
-4.03 |
| 6 |
Fournier, Nicholas A (17)
|
NEGS |
27.70 |
28.24 |
0.54 |
| 7 |
Wolfe, Ezekiel K (16)
|
VAGATR |
30.86 |
28.53 |
-2.33 |
| 8 |
Romero, Richard E (18)
|
PVHACC |
32.21 |
28.93 |
-3.28 |
| 9 |
Woods, Connor E (16)
|
VAGATR |
38.56 |
29.00 |
-9.56 |
| 10 |
Easter, Zachary D (15)
|
VAGATR |
32.83 |
29.07 |
-3.76 |
| 11 |
Gervase, Shaun J (16)
|
VACVAC |
38.80 |
29.28 |
-9.52 |
| 12 |
Kvasov, Ivan (13)
|
NEGS |
34.50 |
31.01 |
-3.49 |
| 13 |
Kobzar, Nikita (14)
|
VACVAC |
36.54 |
31.48 |
-5.06 |
| 14 |
Romero, Elioenai R (15)
|
PVHACC |
|
33.59 |
- |
|
Bolz, Andrew R (16)
|
PVNCAP |
35.32 |
NS |
- |
|
Hayburn, Patrick G (18)
|
MDASC |
|
NS |
- |
|
Folger, Samuel J (18)
|
NEGS |
26.19 |
NS |
- |
|
Couture, Radomyr A (16)
|
VACVAC |
27.98 |
NS |
- |