| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mueller, Jackson S (14)
|
VANOVA |
23.52 |
23.41 |
-0.11 |
| 2 |
Silva, Pablo C (14)
|
VANOVA |
24.98 |
23.72 |
-1.26 |
| 3 |
Kojanov, Mikhail I (13)
|
PVNCAP |
24.88 |
23.87 |
-1.01 |
| 4 |
Thornton, Christian B (14)
|
VANOVA |
23.80 |
24.10 |
0.30 |
| 5 |
Mayr, Charlie T (14)
|
VANOVA |
23.71 |
24.18 |
0.47 |
| 6 |
Dickerson, Jack R (13)
|
VANOVA |
23.95 |
24.37 |
0.42 |
| 7 |
Conner, Joseph H (14)
|
VANOVA |
24.99 |
24.39 |
-0.60 |
| 7 |
King, Michael J (14)
|
VANOVA |
24.43 |
24.39 |
-0.04 |
| 9 |
Nuckols, Cole B (14)
|
VANOVA |
24.53 |
24.74 |
0.21 |
| 10 |
Galyen, Austin M (13)
|
VANOVA |
25.20 |
24.90 |
-0.30 |
| 11 |
Leachman, Scott T (13)
|
VANOVA |
26.03 |
24.95 |
-1.08 |
| 12 |
Causey, Michael R (13)
|
VANOVA |
24.98 |
25.05 |
0.07 |
| 13 |
Smith, Ryan M (13)
|
VANOVA |
25.89 |
25.58 |
-0.31 |
| 14 |
Julian, Bryant M (14)
|
VANOVA |
26.31 |
25.85 |
-0.46 |
| 15 |
Kennedy, Ethan J (14)
|
VANOVA |
25.71 |
26.11 |
0.40 |
| 16 |
Ladman, Micah J (15)
|
VANOVA |
26.67 |
26.47 |
-0.20 |
| 17 |
Green, Brayden J (14)
|
VANOVA |
26.21 |
26.72 |
0.51 |
| 18 |
Meisel, Zachary B (14)
|
PVNCAP |
28.36 |
26.96 |
-1.40 |
| 19 |
LeFaivre, James R (13)
|
PVNCAP |
28.60 |
27.88 |
-0.72 |
|
Olivo Cauberghs, Esteban C (13)
|
PVNCAP |
28.08 |
NS |
- |