| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Samuels, Charlotte G (15)
|
VABASS |
2:33.88 |
2:37.63 |
3.75 |
| 2 |
Fuller, Alexandra G (13)
|
VAACAC |
2:47.37 |
2:38.33 |
-9.04 |
| 3 |
Fleenor, Elizabeth G (14)
|
VAVABR |
2:31.79 |
2:40.70 |
8.91 |
| 4 |
Mizuba, Hannah R (13)
|
VAVABR |
2:47.76 |
2:43.62 |
-4.14 |
| 5 |
Smith, Morgan V (16)
|
VAVABR |
2:38.02 |
2:46.86 |
8.84 |
| 6 |
Ward, Alena F (13)
|
VAVABR |
3:06.36 |
3:01.40 |
-4.96 |
| 7 |
Conforti-Johnson, Sophia K (13)
|
VACCA |
|
3:01.71 |
- |
| 8 |
Hodge, Emma C (15)
|
VACCA |
3:05.14 |
3:06.78 |
1.64 |
| 9 |
Smith, Saige L (13)
|
VABASS |
|
3:32.27 |
- |
|
Lawrence, Peyton E (15)
|
VAVABR |
2:34.94 |
NS |
- |